David Thewlis, Jennifer Jason Leigh and Tom Noonan are to lend their voices in Anomalisa.
The trio will reprise their original stage roles in Charlie Kaufman's upcoming animated short film, reported Screen Daily.
David is set to voice Michael, a motivational speaker who struggles with his inability to connect with people until he meets Lisa (Jennifer). Tom is expected to voice the other characters in the film, as he did in the play.
Charlie, who has adapted the play for the big screen, will co-direct the animated feature with Duke Johnson. It will be his first animated film.
Production on Anomalisa is already under way, and the film will be pitched to buyers at the Cannes Film Festival.
Charlie, who is best known for writing Being John Malkovich, Adaptation and The Eternal Sunshine Of The Spotless Mind, got 406,237 US dollars (around £265,450) worth of financial backing for the project from online crowd-funding site Kickstarter.
David - best known for playing Remus Lupin in the Harry Potter films - will next be seen in Red 2 alongside Bruce Willis, John Malkovich and Dame Helen Mirren, and WikiLeaks film The Fifth Estate with Benedict Cumberbatch.