DJ Dave Lee Travis has been charged with two further offences of indecent assault in addition to 12 counts he already faced.
The 68-year-old has been accused of assaulting a woman aged over 16 between January 1 1992 and December 31 1993, Scotland Yard said.
Travis, of Mentmore, in Leighton Buzzard, Bedfordshire, has already appeared in court to face 12 counts, including indecent assault and sexual assault.
The ex-Radio 1 DJ was first charged on August 15 as part of Operation Yewtree, the police investigation prompted by the Jimmy Savile abuse scandal, but the accusations against Travis have no connection to the disgraced television presenter.
He is due to appear on bail at Westminster Magistrates' Court on Thursday in relation to the new charges.
Travis previously indicated he will plead not guilty in relation to the first 12 charges.
At his last court appearance at the Old Bailey on September 6 , Travis was released on bail on condition that he lives at his Bedfordshire home and does not contact the alleged victims.
Mr Justice Sweeney adjourned the case for a plea and case management hearing on October 21 and fixed a trial date for March 4 next year. The trial is expected to last four to five weeks.