A young mother and a three-year-old girl are seriously ill in hospital after allegedly contracting E.coli from a fish and chip shop.
The Llay Fish Bar on Council Street in Llay, Wrexham, North Wales, is at the centre of the suspected E.coli outbreak.
New mum Karen Morrisroe-Clutton is being treated in Wrexham Maelor Hospital where it is understood she is on a life support machine.
The second victim, who has not been named, is being treated for renal failure at Liverpool's Alder Hey Hospital.
She is related to another person who is ill with the suspected bug, although they are not in hospital.
A fourth person has also been taken ill but is not in hospital. All four are from the Wrexham area.
A spokeswoman for Wrexham Council said they obtained a court order yesterday to keep the Llay Fish Bar closed while further investigations are carried out.
Dr Judy Hart, consultant in communicable disease control at the National Public Health Service for Wales (NPHS), said last night: “The NPHS and Wrexham Council's environmental health officers are investigating four cases of E.coli O157 in three families from the Wrexham area.
“Two people have been hospitalised. As a precautionary measure, a food premises in Llay was closed by the council on July 30 while further investigations are carried out,” Dr Hart added.
“E.coli O157 is a serious bacterial infection that causes abdominal pain and diarrhoea.
“The symptoms of E.coli O157 range from mild diarrhoea, stomach cramps and fever to bloody diarrhoea.
“Most people recover without complications, but the most severe cases can develop kidney failure.”
A spokesman for the North Wales NHS Trust East last night described Mrs Morrisroe-Clutton's condition as “seriously ill”.