People need to decide which side they are on over links with Brussels ahead of next year's European elections, Nick Clegg said as he issued a call to arms for support in the battle against eurosceptics.
Mr Clegg acknowledged a referendum on the UK's membership of the European Union was inevitable, but described the Prime Minister's plan for a renegotiation of the relationship followed by a public vote before the end of 2017 as "flawed".
He said there was a consensus around the need to protect the single market but claimed that any reform deal David Cameron could strike if the Tories are returned to power in 2015 would not be enough to placate those in his party who were committed to a British exit.
The Deputy Prime Minister appealed to business leaders and charities to speak out in favour of the EU before it is too late and Britain finds it has "stumbled out" - a move which would be "economic suicide".
He has written to more than 100 organisations, including business and trade groups and charities, pleading with them to speak out in support of the EU.
Answering questions following a speech at the Buhler Sortex factory in east London, Mr Clegg acknowledged there was common ground with Mr Cameron over the need to protect the UK's interests in the single market as the eurozone countries integrated more closely.
"I don't think it should be any surprise that there is a consensus, other than among those who want to leave the European Union, that the single market is a good thing and needs to be protected," he said.
"Clearly, any further evolution in the way that the European Union is run, not least as the eurozone integrates further, needs to be done in the way which nonetheless safeguards the virtues of that single market stretching across both eurozone-ins and eurozone-outs. I think that is something which I suspect everybody would agree upon."
But Mr Clegg said his coalition partner's strategy for a renegotiation of the relationship with Brussels was doomed because it would not be accepted by his party.
Characterising the plan, with its 2017 deadline, as a political fix to end internal Tory divisions in the run-up to the 2015 general election, Mr Clegg said the Prime Minister was "playing with fire" and "it is Britain that will get burned".
He added: "I don't think it's very complicated to imagine circumstances in which you could reform here, have a little smidgen of repatriation there, a treaty wording change there.
"That's not really the issue. The issue is I don't believe that will ever meet the test which is actually being set by the vast part of the Conservative Party, which is that they don't just want another treaty change with a bit of reform, they want a complete semi-detachment or indeed total exit of the European Union.
"That's why I think the whole strategy is flawed, because it is raising expectations, certainly among the ranks of the Conservative party, which will not be met by that process."
Mr Clegg believes next year's European elections will be fought as a proxy for the choice between remaining in or leaving the 28-member block.
Most European elections have been a "mid-term plebiscite on the performance of the government of the day" but this contest would be more "fundamental".
He said: "No w is the time to have a much more fundamental and visceral debate about what side are you on in this basic divide - are you basically for leading the reform of the European Union by being committed to it, or are you half out the door, or do you advocate exit altogether."
Mr Clegg said among the reforms he would like to see would be an end to the "bonkers" situation where the European Parliament alternates between Brussels and Strasbourg.
"Some of its working practices, not least its second seat in Strasbourg, are totally bonkers and should be changed," he said.
Matthew Elliott, chief executive of campaign group Business for Britain, said: "Nick Clegg has shown today that he is out of touch with how the majority of the British people, including thousands of British business people, feel about the European Union.
"If he thinks that the EU Parliament alternating between Brussels and Strasbourg is the only 'bonkers' aspect that needs changing, he should go and speak to the bosses at companies across Britain, drowning in a sea of absurd EU directives and regulations.
"UK businesses are looking for a proper renegotiation, along the lines set out by the Prime Minister in January, that incorporates treaty change and the repatriation of powers from Brussels.
"Contrary to what he said in this speech, it is the Deputy Prime Minister rather than David Cameron who is 'playing with fire'. By writing off the process of renegotiation before it has even properly begun, Nick Clegg is increasing the likelihood of Britain voting to leave."
But TUC general secretary Frances O'Grady praised Mr Clegg for highlighting some of the workplace rights that are enshrined in EU law.
She said: "Nick Clegg is right to make the case for the EU and say that paid holidays and other workplace rights are some of the best arguments for EU membership. Leaving the EU would be disastrous for jobs and investment.
"His views are in stark contrast with the Prime Minister who wants to offer the dismal choice between leaving the EU or staying in a Europe stripped of rights at work.
"Europe needs to win back popular support, replacing austerity with a people's plan for jobs-led growth and higher living standards. It needs to go back to its roots by balancing a dynamic economy with social protection and fair shares."