Ireland boss Giovanni Trapattoni was dealt a major blow on Sunday when skipper Robbie Keane was forced to withdraw from the squad for the World Cup qualifier against Austria.
The 32-year-old underwent a scan on the calf strain he suffered during Friday's 0-0 draw in Sweden and the results have confirmed that he will be unable to play on Tuesday evening.
Wolves frontman Kevin Doyle, who was informed by text that he had not made the final 23 for the double-header, has been drafted in as a replacement.
A spokesman for the Football Association of Ireland said: "The Football Association of Ireland today confirmed that Robbie Keane has been ruled out of Tuesday's FIFA World Cup qualifier against Austria in Aviva Stadium.
"A scan this morning confirmed that Robbie is suffering from calf strain after Friday's game against Sweden. Wolves striker Kevin Doyle has been called up in place of Keane.
"The manager has spoken to Kevin, and he will link up with the squad in time for training in Malahide tomorrow morning."
Trapattoni had earlier revealed that Keane has gone for a scan, but at the time, was hopeful the Los Angeles Galaxy striker, who was in line to win his 124th cap against the Austrians, would make the game.
The 74-year-old Italian now faces something of a selection headache with Jon Walters also struggling with a back injury and Doyle still coming to terms with his initial disappointment.
Trapattoni has been impressed with Derby's Conor Sammon and has handed Simon Cox key roles in the past, while Wes Hoolahan has impressed in a withdrawn role since being drafted into the squad.