Any remaining hope Reading had of beating the drop appeared to be extinguished as Nigel Adkins' first home match at the helm ended in a 2-0 defeat to former club Southampton.
Goals from Jay Rodriguez and Adam Lallana proved the difference at the Madejski Stadium as Saints ran out comfortable winners, condemning the bottom-placed Royals to a seventh successive defeat.
The result leaves the Berkshire side seven points adrift of safety with just six matches remaining, making an immediate return to the npower Championship increasingly likely.
Talk in the build-up was about Adkins exacting revenge on the club that so harshly disposed of his services 78 days ago, despite having led Saints to back-to-back promotions. However, after weathering the Royals' early pressure, it was successor Mauricio Pochettino that enjoyed the spoils as Saints won a third successive Premier League match for the first time since 2003.
As Reading upped the ante Adam Le Fondre was guilty of wasting a decent effort, blazing over after Saints' attempts to clear a free-kick saw the ball land kindly for him. Adkins' side returned to the attack and felt they should have had a penalty, only for referee Mike Jones to wave away the appeals that followed Jos Hooiveld's challenge on Hal Robson-Kanu.
The opener in the 34th minute owed as much to poor defending as fine attacking play, with an impressive team move ending with Rodriguez playing a one-two with Rickie Lambert before bundling home through Adam Federici's legs. The Australian goalkeeper should have done better and was beaten by Rodriguez again, only for his strike to rattle the crossbar.
Rodriguez has shone since Pochettino's arrival on the south coast and that confidence was epitomised two minutes from half-time, showing incredible pace to burst past the Reading backline. Federici did well to save the striker's right-footed shot when clear on goal, with Ramirez putting the follow up just wide.
Much like the opening period, the hosts were again quicker to settle but failed to turn possession into clear-cut opportunities. That allowed Saints to return to the attack and, after Federici easily dealt with a tame Steven Davis effort, a fine driven Rodriguez cross just evaded Lambert at the far post.
Le Fondre struck wide as Reading searched for an equaliser, although Saints were still looking dangerous themselves. After striking an audacious 25-yard effort wide, Rodriguez put through substitute Lallana to slot home at Federici's near post to send the 2,216 visiting fans into raptures.
It was no less than Pochettino's side deserved but Reading thought they had pulled one back through Le Fondre. Boruc spilled the former Rotherham forward's header but was adjudged to have stopped the ball crossing the line, much to the home fans' annoyance. Saints substitute Guly do Prado should have found the target in stoppage time.