Austrian Jurgen Melzer accused umpire Magdi Somat of being "cocky" after he was issued with a court violation for racquet abuse.
After losing the first set, Melzer and Blake then complained that the umpire was ignoring audible let calls.
The duo lost the match 5-7 0-6 7-6 4-6.
Meanwhile, India's Rohan Bopanna sealed a spot in his first ever Wimbledon semi-finals with French partner Edouard Roger-Vasselin. The 14th seeds knocked out sixth seeded Swede-Canadian combination of Robert Lindstedt and Daniel Nestor with a 7-5, 7-6(3), 6-7(4), 6-7(3), 6-2 win.
Kyle Edmund sees off good friend O'Mara
Kyle Edmund earned the bragging rights from his all-British showdown with friend Jonny O'Mara as he reached the Wimbledon boys' quarter-finals.
The 18-year-olds, the last remaining British juniors in the singles, are long-time friends, having met an under-12s tournament in Corby.
Beverley-based Edmund has even been giving Arbroath's O'Mara a lift to Wimbledon this week, with the latter posting a photo on Twitter of this morning's journey saying the "banter's flying around".
There has been verbal jousting in the build-up as well, with the Scottish wild card gleefully pointing out that he won their only ever previous meeting.
Leander Paes has an up and down sort of a day
In the mixed doubles, 15th seventh seeds Paes and Zheng, lost 3-6, 3-6 to unseeded American-French combo of Eric Butorac and Alize Cornet.
The eighth-seeded Indo-Austrian pair fought their hearts out before bowing out with a 6-7 (5), 6-7 (3), 6-7 (4). Sania Mirza made it to the pre-quarterfinals of the mixed doubles with Horia Tecau beating Martin Emmrich and Julia Goerges 6-3, 6-4.
However, Andy Murray's sometime practice partner Edmund made amends by winning the last-16 clash 6-3 6-2 on Court 14.