Gloucester fell to an agonising 21-17 defeat at Stade Ernest-Wallon as Toulouse pounced with a late try to snatch victory in the opening round of the Heineken Cup.
The Cherry and Whites had looked destined to seal an historic win in Toulouse after tries from Henry Trinder and Charlie Sharples, but the aristocrats of Europe pounced in the dying stages through Clement Poitrenaud.
The build-up to the game had surrounded Mike Tindall's omission from England's Elite squad and £25,000 fine for misdemeanours at the World Cup, but he produced a barnstorming performance and had a hand in both Gloucester tries.
However, it was not enough and the defeat leaves Gloucester third in their pool and with just a losing bonus-point ahead of Saturday's clash with Harlequins at Kingsholm.
Timoci Matanavou also crossed for Toulouse with Luke McAllister kicking two penalties and Lionel Beauxis a penalty and conversion. Freddie Burns kicked a penalty and two conversions for the visitors.