Queen's show a superior class to preserve All-Ireland League 2A status
City of Derry, Rainey and Dungannon failed to finish in the top 10 of 16 teams in 2A so under the new AIL restructure, they have been relegated to 2B.
Each of the new five divisions will have 10 teams and Queen's and Banbridge have managed to retain their 2A status.
Bann finished in fourth but a few weeks ago were challenging for one of the two automatic promotion places to 1B.
Queen's occupied one of those at the end of January before falling away.
Director of Rugby Robbie Moore said: "The fact that we were without so many representative players meant that for most of the season we were not at full strength."
Saturday's 55-26 win over Barnhall ensured their 2A status. Queen's had seven tries with Conor Shiels scoring four, Damon Hall two and one from Jack Owens for Justin Rea to convert six and add a penalty, while Ali Wright contributed a penalty and a conversion.
But it was not Barnhall's day as they were relegated on points difference.
Other results: 2A Banbridge 29 Old Wesley 24, Dungannon 37 Bective 28, Seapoint 12 City of Derry 27, Nenagh/Ormond 26 Rainey 14. 2B Tullamore 0 Armagh 21, DLSP 41 Ards 0.