Tommy Bowe has been ruled out of contention for Ireland’s RBS Six Nations clash with France on Sunday.
Bowe, who missed Saturday's 13-11 victory over Italy, has been omitted from an extended squad as he continues his rehabilitation from a knee injury.
Number eight Jamie Heaslip, flanker Stephen Ferris and winger Andrew Trimble have been added to a revised 33-man party, however.
Heaslip is making progress with his ankle injury and could feature against the Grand Slam champions at Lansdowne Road.
Ferris and Trimble will have their respective knee and hand injuries assessed to decide whether they can participate.
An additional eight players have been called into the squad to supplement the 22 on duty at Stadio Flaminio — Brett Wilkinson, Mick O'Driscoll, Donnacha Ryan, Mike McCarthy, Nevin Spence, Johne Murphy, Andrew Trimble and Gavin Duffy.
Meanwhile, Andy Powell has been ruled out of Wales' clash against Scotland at Murrayfield on Saturday.
The Wasps number eight suffered a shoulder injury during the 26-19 loss to England in Cardiff three days ago, but the Wales camp hope Powell will be back in contention to face Italy later this month.
Wing Morgan Stoddart, meanwhile, has been given extra time to prove his fitness ahead of the Scotland trip after breaking a bone in his hand against England.
Stoddart, Wales' try-scorer at the Millennium Stadium, will be monitored daily to assess his progress.
Cardiff Blues wing Chris Czekaj, meanwhile, has been called into the squad as cover.