Frans Steyn has been ruled out of next week's Tri-Nations encounter against New Zealand in Johannesburg due to injury.
The Racing Metro full-back, one of three fresh faces added to an expanded Springbok squad for the home leg of the competition, has been advised not to train until next Wednesday.
He suffered an injury to his quadriceps in a pre-season match last Friday.
Andy Marinos, the South African Rugby Union (SARU) manager of national teams, confirmed the news, adding that discussions had now been opened with Steyn's club and the French Rugby Federation to secure his availability for the world champions' Tests against Australia on August 28 and September 4.
"The injury situation has to be dealt with first, but we are hopeful that Frans will be available to us after the All Blacks match," Marinos said.
Steyn, who has been capped 37 times, last played for his country in the one-off Test against Wales in Cardiff at the start of the season.
The current 29-man training squad will be trimmed to 24 at the end of a four-day camp on Friday, with the matchday 22 due to be named on Tuesday.