Wales full-back Morgan Stoddart has suffered a suspected broken leg and is expected to miss the World Cup.
Stoddart was carried from the pitch during the 23-19 defeat to England at Twickenham and Wales coach Warren Gatland confirmed the injury appeared serious.
He said: "It looks like a bad break, it's a shame for him."
Gatland confirmed Stephen Jones, who had been due to become his country's most capped player by making a 101st Test appearance until he was withdrawn following the warm-up, had suffered a calf muscle injury.
Gatland said: "He had a tight calf before the game and we didn't want to take any chances.
"If he had torn the calf that is six weeks out."
Stoddart only started at full-back after Jones pulled out shortly before kick-off, forcing Gatland to move to Rhys Priestland into the fly-half jersey. The injury occurred when he was tackled by Delon Armitage having just intercepted a pass from the England wing.
"You have got to feel for him (Stoddart)," said Gatland.
"We have been joking in the past, calling him 'Glass' and 'Crystal' because he does pick up a few injuries but to get an injury like that...you hope he recovers as quickly as possible.
"He did look dangerous with the ball in hand, he looked threatening. It was a shame he picked up that injury."