Uncapped Gloucester centre Billy Twelvetrees will make his England debut in Saturday's Calcutta Cup game against Scotland after being selected in place of the injured Manu Tuilagi.
Twelvetrees will become the 18th new player capped by head coach Stuart Lancaster in 13 Tests when he lines up alongside Brad Barritt in midfield for the RBS 6 Nations opener.
Barritt will revert to the outside centre role he filled on his own Test debut in last year's Calcutta Cup win at Murrayfield, with Twelvetrees operating on his inside.
Joe Marler has returned at loosehead prop in place of Alex Corbisiero, who has been ruled out of the Six Nations with a knee problem.
Other than the two injury-enforced changes, Lancaster has retained the starting line-up that laid the foundations for England's record 38-21 victory over New Zealand two months ago.
That means Tom Youngs keeps the hooker's jersey ahead of the more experienced Dylan Hartley, who missed the autumn series through injury. Ben Youngs has held off the challenge from Danny Care to keep hold of the scrum-half position, and Alex Goode returned from a shoulder injury in time to retain the full-back berth.
"We are happy to stick with the team that did so well against New Zealand, especially in light of the fact that they have all been in good form for their clubs since then," Lancaster said. "There were some very tight calls, with some experienced players coming back into contention, and I am sure the bench will have a significant impact on the game."
Leicester fly-half Toby Flood, the most experienced player in the 23-man squad with 53 caps, comes onto the bench along with Saracens wing David Strettle.
England team: A Goode (Saracens); C Ashton (Saracens), B Barritt (Saracens), B Twelvetrees (Gloucester), M Brown (Harlequins); O Farrell (Saracens), B Youngs (Leicester), J Marler (Harlequins), T Youngs (Leicester), D Cole (Leicester), J Launchbury (Wasps), G Parling (Leicester), T Wood (Northampton), C Robshaw (Harlequins, capt), B Morgan (Gloucester).
Replacements: D Hartley (Northampton), D Wilson (Bath), M Vunipola (Saracens), C Lawes (Northampton), J Haskell (Wasps), D Care (Harlequins), T Flood (Leicester), D Strettle (Saracens).