Wales are set to release updated details of Morgan Stoddart's World Cup-ending injury on Monday.
The Wales players will begin preparations for next Saturday's Millennium Stadium rematch against England without the popular Scarlets back.
Stoddart, 26, broke his left leg during Wales' 23-19 defeat at Twickenham on Saturday and is expected to face a lengthy spell out of the game.
His absence will be a major blow for Wales coach Warren Gatland, who was unable to pick injured full-back Lee Byrne (knee) and wing Leigh Halfpenny (ankle) against England.
Stoddart scored two tries from three starts during last season's RBS 6 Nations Championship, and he was expected to feature among the 30-man World Cup squad Gatland will announce two weeks' today.
Stoddart suffered the injury when he was tackled by England full-back Delon Armitage, having just intercepted a pass, and the extent of it quickly became clear.
Wales centre Jonathan Davies, a Scarlets colleague of Stoddart's, added: "It is terrible news. I am gutted for him because he has worked so hard to get here in the last couple of months.
"I know rugby is a hard game, but when you see a friend that you play rugby with every day pick up an injury like that, it is tough to take.
"I just wish him a speedy recovery and hope he gets back to full fitness."