David Cameron and John Bercow have been threatened in a video released by Islamic State's propaganda wing.
Images of the Prime Minister and the Commons speaker appear in the 17 minute long broadcast posted online by the terror group.
The video appears to feature scenes of the terrorists behind the Paris attacks, including Bilal Hadfi and Samy Amimour, but has only now been published.
Mr Bercow's face appears on screen with a crosshair superimposed over him.
After images of London flash up, footage of the Prime Minister appears with a message warning non-believers will be targeted.
The terror spree last November left 130 dead as IS killers rampaged across the French capital.
Suicide bomber Hadfi was one of three who attacked the Stade de France and was believed to have fought with IS in Syria.
Amimour, also known as Abu Qital al-Faransi, was one of the suicide bombers who blew himself up at the Bataclan music hall. The Frenchman was known to French intelligence services. They were among nine of the suspected attackers killed after carrying out the atrocity.
Officials said the footage was "another desperate move" by IS, also known as Daesh.
A Government spokesman said: "We are currently examining this latest Daesh propaganda video - another desperate move from an appalling terrorist group that is clearly in decline."