Anti-government protesters filled the streets of Venezuela's capital and other major cities in a show of force against President Nicolas Maduro.
Tens of thousands of demonstrators shut down the main highway in Caracas, and police clashed with protesters in other cities in what opposition leaders called "the takeover of Venezuela".
Nationwide at least 140 people were detained by police, according to the Foro Penal human rights group. A police officer was shot and killed, and two others injured, under unclear circumstances in central Miranda state.
"Maduro has shown how scared he is that the people will express themselves," opposition leader Henrique Capriles said.
The protests come after electoral authorities blocked a recall campaign against Mr Maduro last week. The face-off escalated on Tuesday when the opposition-led legislature voted to put the president on trial, accusing him of effectively staging a coup.
Opposition politicians argued that Venezuela's leader has effectively abandoned the presidency by neglecting his job.
Many Venezuelans blame him for the country's triple-digit inflation, free-falling economy and shortages of food, medicines and other basic goods.
Government supporters staged a much smaller rally attended by Mr Maduro in central Caracas.
Opposition leaders ended Wednesday's national day of protest with call for a general strike on Friday. They also threatened to march on the presidential palace in the heart of the city on November 3 if the government does not reverse its decision to block the recall effort.
The opposition has not been allowed to protest in front of the presidential palace since a massive march there helped precipitate a short-lived coup against former president Hugo Chavez in 2002.
On Wednesday, police fired tear gas and clashes with police in provincial capitals that left several wounded. In the border state of Tachira, the windows of the heavily-guarded regional electoral office were broken and anti-government slogans spray-painted on the entrance.
In a video widely circulating on social media, a young man shouted in the face of soldiers in riot gear maintaining a line against a crowd of masked protesters.
"I'm going hungry! If you're going to shoot me because I'm hungry, shoot me," the protester said.
In Caracas, students casually sat on the country's main road. One protester dressed as Lady Justice, with a scale and white blindfold.
Victoria Rodriguez, 18, said she hopes to cast her first vote for the campaign to recall Mr Maduro.
She said she is frustrated that opposition leaders have not called for more dramatic action, like sleeping on the road overnight or attempting to paralyse the capital for days at a time.
"People are tired of going to the streets and then going home," she said. "The opposition is letting the streets go cold. They are giving the government too much time to manoeuvre."
Congress was expected to take up the issue of Mr Maduro's responsibility for the country's worsening political and economic crisis on Thursday. The result of that debate is unlikely to have much impact, however.
Unlike other countries in Latin America such as Brazil, where Dilma Rousseff was removed from the presidency in August, Venezuela's National Assembly cannot impeach the president. That power lies with the Supreme Court, which has never voted against Mr Maduro.
The government and opposition have agreed on an attempt at dialogue to defuse the crisis.
Talks sponsored by the Vatican and other South American governments are set to begin on Sunday in the Caribbean island of Margarita.
Mr Maduro, who met Pope Francis privately at the Vatican on Monday, said he will travel to Margarita to personally launch the talks.
But the two sides have tried dialogue during previous crises, and the opposition has little hope of a breakthrough.
Although Venezuelans overwhelmingly blame Mr Maduro for their economic woes the ruling party is in firm control of institutions like the military and has shown no interest in yielding to the opposition.