Government forces and tribal fighters exchanged gun and artillery fire in Yemen's capital Sanaa on Wednesday morning in fresh fighting that killed at least 41 people.
The fighting spread to new areas, with tribesmen from the powerful Hasid confederation seizing buildings in neighbourhoods in the city's south and north-west.
The urban battles over the last week have posed a new threat to President Ali Abdullah Saleh's 33-year rule.
For nearly four months, thousands of Yemenis have filled the streets daily, calling for democratic reforms and Saleh's removal from power.
The mostly peaceful protests gave way last week to violence between Saleh's security forces and fighters loyal to Sheik Sadeq al-Ahmar, head of the country's largest tribal coalition.
Fighting in Sanaa raged until 5am, and witnesses said units of the elite Presidential Guard, commanded by one of Saleh's sons, shelled the headquarters of an army brigade responsible for guarding sensitive government institutions.
Army officers who have defected to the opposition said the government suspected the brigade commander was about to join forces with the movement to oust Saleh.
Opposition army officers said the armoured brigade commander, Brigadier-General Mohammed Khalil, was neutral and without political affiliation but had apparently angered Saleh.
The 41 dead included combatants from both sides of the conflict, said medical officials.
The fighting has engulfed the northern Hassaba neighbourhood, where tribal fighters have seized a number of government ministries and buildings. Government artillery fire has heavily damaged the house of al-Ahmar, the tribal leader, and the government has cut the area's electricity and water supplies.