Italy centre Luca Morisi has been ruled out of the Rugby World Cup because of a torn ligament in his right knee.
The 20-year-old Treviso player suffered the injury during the friendly defeat against Wales in Cardiff on Saturday.
Gonzalo Garcia picked up an ankle problem against the Welsh, but is expected to continue his rehabiliation with the rest of the squad.
Captain Sergio Parisse, however, will stay at French club Stade Francais after having fluid drained from his left leg before heading to England.
Italy start their World Cup campaign on September 19 at Twickenham against France in Pool D.