Finn Russell will not be involved in Scotland's Guinness Six Nations clash against England on Saturday.
The Racing 92 fly-half missed Scotland's 19-12 defeat to Ireland in Dublin last Saturday after being disciplined for breaching team rules last week following an alleged late-night drinking session at the Dark Blues' team hotel.
Adam Hastings filled the fly-half role in Dublin, and Scotland's 37-man squad for England's Murrayfield visit also includes Worcester No.10 Duncan Weir, who was called up last week in place of Russell.
But there is no recall for 27-year-old playmaker Russell in head coach Gregor Townsend's latest group.
It will inevitably raise further questions about whether he will be involved at all for the Dark Blues in this season's tournament.
Scotland will also be without wing Darcy Graham again, who has a knee injury.
England lost on their last Calcutta Cup visit to Murrayfield in 2018, and they will arrive in the Scottish capital following a 24-17 defeat against France last Sunday.
Squad: Backs: C Harris (Gloucester), A Hastings (Glasgow), S Hogg (Exeter, capt), G Horne (Glasgow), R Hutchinson (Northampton), S Johnson (Glasgow), H Jones (Glasgow), B Kinghorn (Edinburgh), S Maitland (Saracens), B McGuigan (Sale Sharks), A Price (Glasgow), H Pyrgos (Edinburgh), M Scott (Edinburgh), K Steyn (Glasgow), R Tagive (Glasgow), D Weir (Worcester).
Forwards: S Berghan (Edinburgh), J Bhatti (Edinburgh), M Bradbury (Edinburgh), F Brown (Glasgow), A Craig (Gloucester), L Crosbie (Edinburgh), S Cummings (Glasgow), A Dell (London Irish), C Du Preez (Worcester), Z Fagerson (Edinburgh), G Gilchrist (Edinburgh), T Gordon (Glasgow), J Gray (Glasgow), N Haining (Edinburgh), S McInally (Edinburgh), W Nel (Edinburgh), J Ritchie (Edinburgh), R Sutherland (Edinburgh), B Toolis (Edinburgh), G Turner (Glasgow), H Watson (Edinburgh)