Andy Murray will be the No 3 seed at Wimbledon while Rafael Nadal has dropped to 10th, it was announced yesterday.
Murray is gunning for his second Wimbledon title at the All England Club after becoming the tournament's first British men's singles champion since 1936 two years ago.
The Scot will be keen to steer clear of Nadal in tomorrow's draw, however, given the Spaniard could meet any of the top four seeds as early as the quarter-finals.
Nadal was seeded second at Wimbledon last year but a lack of form and fitness has seen the 14-time Grand Slam champion tumble down the ATP rankings.
World No 1 Novak Djokovic, who beat Nadal in the French Open quarter-finals three weeks ago, is seeded first while Roger Federer, bidding for a record eighth singles crown at SW19, is second.
Stan Wawrinka, fresh from securing his second Grand Slam title in Paris, completes the men's top four.
Lower down, Murray's conqueror last year, Grigor Dimitrov, is the No 11 seed while the likes of Ivo Karlovic (23), Nick Kyrgios (26), Bernard Tomic (27), and Jack Sock (31) all represent dangerous opponents on grass.
In the women's draw, Serena Williams is the top seed as she looks to seal her sixth singles title at Wimbledon and her 21st Major in all.
Defending champion Petra Kvitova, who pulled out of the Aegon International at Eastbourne on Tuesday with illness, is second, while Romanian Simona Halep is third and Maria Sharapova fourth.
Britain's Heather Watson misses out on a seeding after her WTA ranking dropped to 64.
Laura Robson has been handed a wildcard after she recently returned from a 17-month absence with injury.
Meanwhile, Gilles Simon will tackle Sam Querrey in the quarter-finals of the Aegon Open after easing past Joao Sousa yesterday.
The French World No 13, seeded third, needed only an hour and 12 minutes to beat his Portuguese opponent 6-4 6-1 in Nottingham, while 12th seed Querrey downed Pablo Cuevas 7-6 (9-7) 7-6 (7-3).
Leonardo Mayer, the fourth seed, survived a spirited examination from Czech left-hander Jiri Vesely to progress 7-6 (7-2) 7-6 (7-5) and he will now tackle Denis Istomin for a semi-final place.
The Uzbek fired down eight aces as he defeated Sergiy Stakhovsky 7-5 6-4.
Simone Bolelli emerged victorious from an all-Italian encounter with eighth seed Adrian Mannarino, who succumbed to a 3-6 6-4 6-4 loss.
Former Australian Open finalist Marcos Baghdatis awaits the Bolognese player after conquering Alexander Zverev 6-3 6-3.
Elsewhere, Ukrainian hopeful Alexandr Dolgopolov removed seventh seed Dominic Thiem from contention by clinching a 6-3 6-3 win in only 50 minutes.
Great Britain's Jo Konta has entered uncharted territory at Eastbourne after beating Garbine Muguruza to reach the quarter-finals.
The 24-year-old, in the Aegon International on a wild card, removed World No 8 Ekaterina Makarova on Tuesday and followed up that scalp with a 6-4 4-6 6-3 defeat of 14th seed Muguruza.
She will next face Swiss teenager Belinda Bencic, who was leading Eugenie Bouchard 6-4 3-0 before the Canadian retired hurt from their third-round clash at Devonshire Park.
Australia-born Konta is ranked 146th in the world and has been handed a wild card into Wimbledon, which begins on Monday.