Kyrgios is in hot water over his umpire outburst
Nick Kyrgios lost 6-7 (3) 7-6 (4) 6-2 to Russian Karen Khachanov at the Western and Southern Open in Cincinnati - and also lost his temper with the umpire.
The mercurial Australian unleashed a profanity-laced tirade at chair umpire Fergus Murphy.
Kyrgios won the opening set despite foot and ankle issues, but after a rant he threw his towel over a camera, forcing the broadcasters to switch to an overhead view.
After Khachanov won the second set, Kyrgios shouted: "The worst f*****g ref ever, the worst ref in the game.
"The worst, hands down. Every time I play he's doing some stupid sh*t, every time."
At the end of the match Kyrgios unleashed on Murphy again, saying: "You're a f*****g tool bro."
Khachanov now moves on to the third round and will take on Frenchman Lucas Pouille, who beat Canadian Denis Shapovalov 6-4 6-4.
Kyrgios is likely to face disciplinary action for his outbursts.
Earlier, Alexander Zverev hit 20 double faults in a bruising loss to 19-year-old qualifier Miomir Kecmanovic, who benefited from his opponent's wayward serving in a 6-7 (4) 6-2 6-4 win.